Density Gradient Centrifugation Alone or the Combination of DGC with Annexin V Magnetic-Activated Cell Sorting Prior to Cryopreservation Enhances the Postthaw Quality of Sperm from Infertile Male Patients with Poor Sperm Quality
نویسندگان
چکیده
Objective. To examine whether density gradient centrifugation (DGC) alone or its combination with annexin V magnetic-activated cell sorting (DGC-MACS) can be used to process semen samples from infertile male patients poor sperm quality prior subjecting these freeze/thaw in order optimize the outcomes of freezing. Methods. This study enrolled sixteen concentration ≥ 20 × 10 6 / mL , id="M2"> motility < 30 %, and/or <4% normal morphology. Sperms were processed by DGC DGC-MACS process. Sperm motility, hyperosmotic swelling test (HOS), TUNEL test, and morphological analysis performed before after Results. The had a detrimental effect on viability, morphology, DNA integrity all three groups (RAW, DGC, + MACS groups). showed increased morphology following than untreated frozen controls. motility viability not significantly different between DGC-MACS-CPT (cryopreservation-thawing) DGC-CPT groups. Moreover, almost no grade A B was observed selected decreased levels fragmentation RAW freeze/thaw. group lower that group. Conclusions. preparation cryopreservation improved postthaw males quality. If is foreseen insufficient for artificial insemination husband’s vitro fertilization, if there high sperm, should reduce improve available intracytoplasmic injection.
منابع مشابه
Sperm selection using single layer centrifugation prior to cryopreservation can increase thawed sperm quality in stallions.
REASONS FOR PERFORMING STUDY The increasing use of modern reproductive techniques in human medicine has led to a higher demand for isolation of motile sperm. Several of these isolation techniques have been adapted for veterinary use and can be applied for the selection of a superior sperm sample from stallion semen. Until recently a major disadvantage of such isolation techniques was the limita...
متن کاملP-58: Which Novel Sperm Selection is Suitable for ICSI? MACS-DGC or DGC-MACS
Background: Super-paramagnetic annexin-V conjugated micro-beads are able to eliminate sperm with phosphatidylserine externalization (PSE). PSE is considered as apoptotic sperm. PES positive sperm can be removed from sperm preparations sample to enhance sperm quality for assisted medical procreation. Therefore, the efficiency of this technique over normal sperm processing procedure such as densi...
متن کاملEvaluation of sperm recovery following annexin V magnetic-activated cell sorting separation.
Magnetic-activated cell sorting (MACS) using paramagnetic annexin V-conjugated microbeads eliminates spermatozoa with externalized phosphatidylserine, which is considered one of the features of apoptosis. The objective of this study was to evaluate sperm recovery following the use of MACS as a sperm preparation technique. Mature spermatozoa were separated and divided into two fractions: the fir...
متن کاملEfficient isolation of sperm with high DNA integrity and stable chromatin packaging by a combination of density-gradient centrifugation and magnetic-activated cell sorting
OBJECTIVE This study was carried out to investigate the correlations of the sperm DNA fragmentation index (DFI) with semen parameters and apoptosis, and to investigate the effects of density-gradient centrifugation (DGC) and magnetic-activated cell sorting (MACS) on reducing the proportion of sperm with DNA fragmentation and protamine deficiency. METHODS Semen analysis and a sperm DNA fragmen...
متن کاملReduction of sperm DNA fragmentation using the magnetic activated cell sorting (MACS) with annexin V microbeads in assisted reproduction
Sperm DNA integrity is an important paternal factor that has been shown to influence fertilization rates, embryo development and pregnancy rates. To reduces the number of apoptotic spermatozoa many technics are used before ICSI treatment ( e.g. using of antioxidants, testicular spermatozoa). Recently, the use of annexin V microbeads, as a non-invasive method to reduce high levels of sperm apopt...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Andrologia
سال: 2023
ISSN: ['0303-4569', '1439-0272']
DOI: https://doi.org/10.1155/2023/9030902